The latest advance in mouse brain imaging comes from Hong Kong University of Science and Technology (), combining three-photon and adaptive optics techniques.HKUST has demonstrated in vivo imaging of ...

We organized the technical workshop on multi-scale optical microscopy for brain imaging in mice and other model organisms. I also gave a talk where I presented some recent results from my lab, on a ...

The ability to perform simultaneous two-photon and three-photon imaging further enhances the system's versatility, allowing detailed exploration of both shallow and deep regions. Read the full ...

More information: Jianan Qu, Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping, Nature Biotechnology (2022). DOI: 10.1038/s41587-022-01343-w.

The impact of two-photon microscopy on functional imaging of neuron activity was recognized when the technique's pioneers were awarded the €1 million Brain Prize in 2015, but certain practical ...

The ability to perform simultaneous two-photon and three-photon imaging further enhances the system’s versatility, allowing detailed exploration of both shallow and deep regions.

To overcome this challenge, the researchers combined STED microscopy with two-photon excitation (2PE) and adaptive optics. "2PE enables imaging deeper in tissue by using near-infrared wavelengths ...