Previous studies have shown a dramatic increase in glucose metabolism in T cells after activation, and the in vitro culture of T cells in the absence of glucose results in cell death within 24 ...

In this article, ACROBiosystems discuss the importance of T cells in the immune response and shares some essential tips and methods for culturing T-cells efficiently and effectively.

The Agilent NovoCyte Quanteon flow cytometer and xCELLigence Real-Time Cell Analysis platform were used to develop a series of in vitro preclinical CAR T-cell safety and efficacy assays.

Alternatively, autologous T cells can be engineered in vitro to become tumor-reactive by the introduction of genes that encode receptors specific for tumor antigens (either T-cell receptors [TCRs ...

Purified CD4+ T lymphocytes obtained from the NHDs PBMC were co-cultured in-vitro with autologous immortalized B cells transfected with CMVpp65 mRNA fused to MITD (IBMAM) as a model antigen and then ...