Timing 2 h. 1. For the expression of human MHC class I heavy chains use the bacterial strain Bl21 (DE3) pLysS. For the production of human β2m use the bacterial stain XL1 Blue.

Major histocompatibility complex (MHC) molecules are known to mediate reverse signaling. Cao and colleagues show that reverse signaling via MHC class I attenuates Toll-like receptor–triggered ...