This protocol describes affinity purification of endogenous protein complexes for mass spectrometry analysis. Optimized to study formaldehyde-crosslinked proteins isolated by chromatin ...

Enriching low-abundant proteins from complex biological matrices is crucial in numerous proteomic workflows. This article outlines an immunoprecipitation method compatible with Quantum-Si’s next ...

This protocol refines co-immunoprecipitation (co-IP) for lipid droplet (LD)-specific protein interactions by isolating ...

An international team led by Einstein Professor Cecilia Clementi in the Department of Physics at Freie Universität Berlin has ...

According to Gilmour, these initial experiments established the feasibility of protein-DNA crosslinks in intact cells combined with immunoprecipitation. “That is helpful to people because it gives ...

After purification, the components of the protein complex can be separated using techniques that include isoelectric focusing, SDS-PAGE, or different types of separation method in 2D.

EPISODE DESCRIPTION. Proteomics - Immunoprecipitation: Understand the significance of proteins in biological research and the role of immunoprecipitation: After listening to the podcast, learners will ...

All the latest science news on chromatin immunoprecipitation from Phys.org. Find the latest news, advancements ... while researching a ternary protein complex in rice nuclei that affects ...

Credit: RENATO PARO" /> Credit: RENATO PAROEarly in the summer of 1991, Valerio Orlando, a postdoc in Renato Paro's lab at the University of Heidelberg, began working on the problem of identifying ...

Uncovering the protein complex critical to male fertility. ScienceDaily. Retrieved July 12, 2025 from www.sciencedaily.com / releases / 2025 / 03 / 250303191303.htm. Osaka University.

A pathway critical for regulating a form of cell death known as necroptosis has been identified. The team's preclinical findings suggest that an inhibitor targeting this PPP13RG protein complex ...