Here, we expand the repertoire of PROTAC compatible E3 ligases by identifying a novel small molecule scaffold targeting the ubiquitin E3 ligase KLHDC2 using a fluorescence polarization-based high ...

Schulich Faculty of Chemistry, the Grand Technion Energy Program and the Resnick Sustainability Center for Catalysis, Technion − Israel Institute of Technology, Technion City, Haifa 3200003, Israel ...

The column was washed with wash buffer (50 mM MES pH 6.0, 6 M guanidine-HCl) and eluted with 50 mM acetate pH 4.0, 6 M guanidine-HCl. The chemokine-containing elutions were pooled and dithiothreitol ...

Department of Chemistry and Biochemistry, Central Michigan University, Mount Pleasant, Michigan 48859, United States Biochemistry, Cell, and Molecular Biology Graduate Programs, Central Michigan ...

Membranes were stained with Ponceau S staining solution for protein loading. Cells expressing the protein of interest were resuspended in a lysis buffer containing 50 mM HEPES (pH 8.0), 300 mM NaCl ...