Mitochondrial reactive oxygen species are required for antigen-specific T cell activation. ... and the in vitro culture of T cells in the absence of glucose results in cell death within 24 hours.

T cell activation plays a key role in the immune response. ... the primary in vitro activation methods of T cells on the market are: ... FGL1 Protein Characterization for Cancer Detection.

The authors transduced primary human T cells from heathy donors with a lentiviral library containing 12,000 bar-coded genes to determine the effect of each gene on T-cell proliferation.

The Agilent NovoCyte Quanteon flow cytometer and xCELLigence Real-Time Cell Analysis platform were used to develop a series of in vitro preclinical CAR T-cell safety and efficacy assays.